In vitro and in vivo wound healing activities of Globulariaarabica leaf methanolic extract in diabetic rats Ahmad Za’al Alsarayreh PhD1Jumah Mutie Shakhanbeh PhD1 Journal of cosmetic dermatology Received: 12 January 2022 | Revised: 17 February 2022 | Accepted: 21 February 2022 Abstract : Aim: This study assessed the effects of topical application of the plant Globularia arabica extract on the diabetic rate of wound closure.Methods: The effect of methanol extracts of G. arabica leaf extract on proinflammatory and anti-inflammatory cytokines, as well as the rate of wound contraction, hydroxyproline, and collagen content, was examined using an excision model ofwound repair in male rats.Results: The healing pattern was also highly related to using 10% plant extract andsignificantly, (p-values <0.025) increased wound contraction and increased hydroxyproline and collagen expression in both non-diabetic and diabetic rats, demonstratingthat G. arabica extract possesses potent wound healing capacity. During the earlywound healing phase, IL-6 levels were found to be upregulated by G. arabica treatment. Increased wound contraction augmented hydroxyproline and collagen content, supporting the early wound healing exhibited by G. arabica. One of the ways forspeeding wound healing may be the induction of cytokine production. The result ofour study shows that the fibroblast cell line treated with 20 µg/ml methanolic extractof G. arabica significantly (p-values <0.035) increases the cell migration that indicatedthe high wound healing activity.Conclusion: Results suggest that G. arabica may be useful in the topical managementof wound healing