Abstract : Background: Vaccines are effective measures that can mitigate the high burden of diseases. However, vaccine refusal poses serious challenges for achieving coverage for population immunity. With the availability of COVID-19 vaccines, limited information is available about the university students’ acceptability and attitudes towards the COVID-19 vaccines in Jordan. Aims: This study aimed to examine the factors that influence university students’ decision of acceptability to COVID-19 vaccine in Jordan. It also examined the impact of perceived risks of the vaccine on students’ decision toward accepting it. Methods: A cross-sectional online survey was conducted among university students from different Jordanian regions. Data collection was carried out using online questionnaire between December 2020 and March 2021. Results: A total of 615 students completed the questionnaire. Students (64.4%) reported that they had adequate information about the vaccination. As well, 45.2% of the students reported that community-based immunity is enough to stop virus spreading indicating that the vaccine is not necessary. Moreover, 65% of students had concerns regarding the vaccine’s adverse effects, safety, and efficacy, which influencing their decision to take the vaccine. Finally, distrust in the local health authorities and lack of transparent and reliable information about the vaccine were observed as factors influencing their decision to take the vaccine. Conclusions: The survey results highlight the continued need for clear and consistent information about the vaccine by healthcare decision makers and university administrations. This information is crucial to reduce the refusal and raise the vaccine’s acceptance.