The current research employed a sample of Jordanian English language and literature university students at Mutah University, in an attempt to examine and analyse the capacity they have got to comprehend and learn English grammatical collocations with prepositions. It also aimed at determining how the students’ L1 (Arabic) affects the acquisition of knowledge of these collocations. For this purpose, the SPSS 22.0 was used in order to analyse data pertaining to grammatical collocations with prepositions, where the data were collected via a collocation test. The findings revealed that the extent of  learning and comprehension of grammatical collocations with prepositions among Jordanian EFL students was found to be relatively inadequate and the most challenging form of collocation was found to be verb + preposition. In addition, a negative effect of the students' native tongue (Arabic) on the learning and use of English grammatical collocations with prepositions was found to be present among the studied sample, as the majority of identified errors arising from L1 interference.