Combined supplementation of soy and garlic modulate biochemical parameters of 7,12-dimethylbenz[α]anthracene induced mammary cancer in female Albino rats

Moayad Khataibeh, Khalid Abu-Alruz, Omar Al-Widyan, Mahmoud Abu-Samak and Jafar Al-Qudah

Abstract:

The study was designed to investigate the chemoprotective effect of Combined Supplementation of soy and garlic on 7,12-dimethylbenz[α]anthrance (DMBA) induced mammary cancer in female Albino rats. Animals (eighty rats) where equally divided into four groups, (twenty rats each). Group I: each rat received 1 mL of 0.1% saline daily for twenty days. Group II: received 1 mL of 0.1% saline given orally once a day for twenty days after DMBA infusion (30 mg kg^-1) and these rats served as controls (cancer control) for groups III and IV. Group III: were post treated with garlic after Infusion with DMBA. Group IV: Treated with Combined Supplementation of Soy and Garlic after DMBA infusion. Biochemical measurement were carried out on liver tissues and serum of experimental animals. Circulating levels of lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT) and glutamate pyrovate transaminase (GPT) were elevated, while the activities of glutathione-s-transferase (GST) and superoxide dismutase (SOD) and the levels of reduced glutathione (GSH) were reduced in liver tissues in DMBA infused rats as compared to normal. Combined Supplementation of Soy and Garlic treatment altered the above mentioned biochemical parameters towards normal values. Present findings indicate that combined supplementation of soy and garlic got more marked effect on DMBA infused rats as compared to a group of rats received only garlic treatment.

DOI: 10.3923/pjbs.2007.2308.2313