The need to find alternatives to existing antibiotic drugs and to cure cancer increases every day. Medicinal plants contain a vast array of substances that indicate antimicrobial and anti proliferative activity. Therefore, antibacterial, antifungal, antioxidant, and anti-proliferative effects of ethanol extracts of Eucalyptus camaldulensis and Pistacia atlantica were evaluated. Antibacterial effect was measured using agar well diffusion and microtiter plate dilution methods. Antioxidant effect was assessed using DPPH and ABTS assays. Anti-proliferative effects were assessed using the MTT assay against K562 leukemia cell line. Eucalyptus camaldulensis fruits and leaves extracts and P. atlantica leaves extract had strong antibacterial activity against Bacillus megaterium, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus aureus, except P. atlantica leaves extract did not have an antibacterial effect against Corynebacterium diphtheria. Pistacia atlantica leaves extract had strong antifungal activity against Aspergillus alliaceus, Aspergillus niger, Rhizoupus stolonifer, and Cunninghamella elegans while it was insensitive against Aspergillus flavus, which was the only tested fungi affected by E. camaldulensis fruit extract. Eucalyptus camaldulensis leaves extract had strong antifungal activity against A. alliaceus. Pistacia atlantica petioles extract had strong cytotoxic activity against K562 cell line while the cytotoxic effect of E. camaldulensis fruits and leaves extracts were moderate. All plant extracts had low cytotoxic effects against normal skin fibroblast cell line. Pistacia atlantica leaves extract had the highest total phenolic content followed by E. camaldulensis leaves extract; then, camaldulensis fruit extract had the highest total flavonoids content followed by P. atlantica leaves extract then E. camaldulensis leaves extract.