Abstract:

  Flavonoids and branched chain amino acids (BCAAs) have been recognized as attractive agents for research due to their role in diabetes and cancer. The present study investigates the antiglycation effect of BCAAs and flavonoid compounds and their cytotoxic effect on different colorectal cancer cell lines individually and in combination. This study was conducted by using RAW 264.7 cell line (murine monocyte-macrophage cell and Methylglyoxal (MGO) for inducing the glycation effect. The Sulforhodamine B (SRB) assay was used to study the cytotoxicity of BCAAs and flavonoids compounds using colorectal cell lines SW480, SW620 and CACO2. Flavonoids showed significant antiglycation effect compared to Aminoguanidine (AMG), while none of the BCAAs compounds displayed antiglycation effect. All flavonoids and branchedchain amino acids showed less degree of cytotoxicity against SW620, CACO2 and SW480 as compared to cisplatin. The synergetic effects was only shown against SW620 and CACO2 cell lines by using the combination of Apigenin and BCAAs while antagonistic effect appeared when combination of Quercetin and Hesperidin were used against SW480, SW620 and CACO2 cell lines. Flavonoids such as Quercetin, Apigenin and Hesperidin could be used as a remedy for diabetes due to their surpassing effect upon antiglycation action of AMG