1. Article title:
Cytotoxic Activity of Methanol Extract of Cynanchum acutum L. Seeds on Human Cancer Cell Lines
2. Article author or authors
Ahmed Mohamed Mohamed Youssef, Yousef Mohammad Al-saraireh, Zeinab El-swaify
3. Name of journal and date of publication
Latin American Journal of Pharmacy (2018/10/1)
4. Article abstract
Cynanchum acutum L., Asclepiadaceae, has been used in the Egyptian folk medicine. HPLC analysis of the extract allowed the identification of 17 phenolic acids and 19 flavonoids. Quantitative analysis of some phenolic acids and flavonoids revealed the presence of vanillic acid (0.781 mg/100 g), benzoic acid (0.590 mg/100 g), pyrogallol (0.325 mg/100 g), catechin (0.32 mg/100 g), acacetin-neorutinoside (1.04 mg/100 g), hesperidin (3.48 mg/100 g), and kaempferol-3-glucoside-2”-p-coumaroyl (5.12 mg/100 g) as major compounds in the extract. The cytotoxic effect of the extract was investigated against colon cancer (Caco-2), breast cancer (MCF-7), hepatocellular carcinaoma (HepG-2), lung cancer (A549), prostate cancer (PC3), human epithelioma cells (Hep-2) and cervical carcinoma (Hela) cell lines. Cell viability was quantitated by MTT assay and IC50was estimated. The IC50values against Caco-2, MCF-7, HepG-2, A549, PC3, Hep-2 and Hela cell lines were determined 3.2, 2.7, 2.3, 2.5, 2.7, 2.6, and 6.6 mg/mL, respectively. Therefore, Cynanchum acutumL. seeds could be considered as a potential chemotherapeutic agent in cancer treatment in future.
