ABSTRACT:

One of the most severe diseases in the world worldwide is prostate cancer in humans. Natural compounds have demonstrated possible anti-cancer properties. One of the potentially therapeutic natural polyphenolic chemicals is rutin. Extracellular vesicles—particularly exosomes—have garnered attention lately due to their potential therapeutic benefits for a variety of chronic illnesses. Exosomes have a variety of functions, such as the ability to stop the growth of cancer cells and trigger apoptosis. In the current in vitro study, the anticancer effect of rutin and exosomes generated from cord blood stem cells (CBSC) on prostate cancer (PC3 and DU145) cells was investigated. The efficacy of rutin and exosome against oxidative stress was assessed using the Comet test, and the expression levels of specific genes, including P53, P21, Caspase3, S100, and BCL2, were evaluated by QPCR. When PC3 and DU145 were exposed to rutin and exosome, there was an apparent increase in DNA damage as compared to the untreated cells. Certain genes showed an increase in expression following both rutin and exosome therapyIt is proposed that the administration of both rutin and exosomes together will cause p53- mediated cell cycle arrest. The combination of rutin and exosome may be able to cause apoptosis more frequently, according to data from both kinds of rutin, exosome, and the combination of both therapies.