Attempts for Detection of Nanoparticles-Nanobacteria and Distribution of their Antibodies in Jordanian Patients with Urolithiasis

JJBS Volume 1, Number 4, December. 2008 ISSN 1995-6673 Pages 147- 152 Jordan Journal of Biological Sciences

 Amin A. aqel

Abstract

In 1998, calcifying nanoparticles (CNPs) or nanobacteria was proposed as an explanation of certain kinds of pathologic calcification and stone formation. In the present study Enzyme Linked Immunosorbent Assay (ELISA), bacterial culturing, and staining techniques were used to investigate the incidence of calcifying nanoparticles IgG antibodies (anti-CNP Abs) in serum of Jordanian patients with urolithiasis and the living nature of these CNPs in the renal stones. Serum samples from 65 patients and related 20 healthy individuals were tested for anti-CNP Abs. Renal stones retrieved from the kidneys of 20 patients were processed and subjected to mammalian cell culture conditions, then bacterial growth and staining were observed from these cultures. Results revealed detection of anti-CNP Abs in 96% of patients and in 40% of healthy individuals. Although high anti-CNP Abs incidence were correlated strongly with the presence of CNPs and urolithiasis, no CNPs or bacterial growth was detected - following the applied staining and turbidity methods, which may reflect the non living nature of such particles. The findings of this study can be used as a tool for early prediction of kidney stone formation.