Asian Journal of Biochemistry

Year: 2012 | Volume: 7 | Issue: 4 | Page No.: 218-225

DOI: 10.3923/ajb.2012.218.225


Formation of Highly Antioxidative Liposomes from Crude Acetone Extracts of Canna indicaCucumis melo andPrunus armeniaca

O.M. Atrooz

Abstract: Liposomes are often used as a carrier to improve the therapeutic activity and safety of drugs. The lipid composition of liposomes plays an important role in determining the behavior of liposomes in phases. In this study, the phospholipid and alkylresorcinol content in crude acetone extracts of seeds of Canna indica L., Cucumis melo L. and Prunus armeniaca L. was analyzed in order to assess the applicability in liposome delivery vesicles. The crude extracts were used to form liposomes and their stability and resistance to oxidative degradation was evaluated by spectroscopy. The liposomes formed from these extracts were stable compared to control Phosphatidylcholine (PC) liposomes and had a lower percentage of aggregation as a function of time, as measured by the Optical Density (OD) at 400 nm (6.1-26.6% for extract liposomes and 43% for PC liposomes). Lipid peroxidation measured by the Thiobarbituric Acid (TBA) method after 28 days incubation at room temperature was much lower for extract liposomes compared to PC liposomes. Interactions of the extract liposomes with human white blood cells resulted in a reduction of the free radical (O2-) formation by 38-65% compared to PC liposomes.