A comparison of cardiac glutathione S-transferases from wild and domestic animals

Abdelrahim A. Hunaiti



1. 1. Cardiac glutathione S-transferases from wild animals; hyena, red fox, porcupine, coypu and mountain gazelle were purified and compared with the enzymes from domestic animals; cow, camel, goat and sheep.


2. 2. By using 1-chloro-2,4-dinitrobenzene as a substrate, domestic hearts expressed higher glutathione conjugating activity than wild animals hearts.

3. 3. In all the studied hearts, the bulk of the activity was associated with near neutral and acidic glutathione S-transferase isozymes with pI values ranging from 4 to 7.4.

4. 4. The enzymes from domestic animals displayed homodimeric structure of 25,000 mol. wt subunit while of the wild animals both hyena and coypu displayed homodimers of 26,500 mol. wt subunit and the rest exhibited heterodimers of 25,000 and 28,000 mol. wt subunits.

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Volume 95, Issue 2, 1990, Pages 275-280